J. Kulys et al., KINETICS OF GLUCOSE-OXIDASE CATALYZED ELECTRON-TRANSFER MEDIATED BY SULFUR AND SELENIUM-COMPOUNDS, FEBS letters, 329(1-2), 1993, pp. 205-209
Unusually high electron transfer rates in Aspergillus niger glucose ox
idase catalyzed oxidation of glucose using 5,6:11,12-Bis(dithio)tetrac
ene (TTT), 1,2-dimethyltetraselenafulvalene (DMTSF) and tetrathiafulva
lene (TTF) were observed. At pH 7.0 oxidation rate constants (TN/K(m))
in the range from 1.0 . 10(7) to 8.7 . 10(7) M . s-1 were deduced fro
m experimental data. One of the investigated mediators, DMTSF, has bee
n used for electrocatalytical glucose oxidation on graphite at a poten
tial of 0.3 V vs. a standard calomel electrode (SCE). The prepared bio
electrodes have a sensitivity of 1.3 muA/(cm2 . mM), a pH optimum at 6
.5-7.0, and a linear range which covers the relevant range for monitor
ing physiological levels of glucose. The bioelectrodes are stable for
more than one month.