METHOD FOR 3D VOLUMETRIC-ANALYSIS OF INTRANUCLEAR FLUORESCENCE DISTRIBUTION IN CONFOCAL MICROSCOPY

Authors
PARAZZA F HUMBERT C USSON Y
Citation
F. Parazza et al., METHOD FOR 3D VOLUMETRIC-ANALYSIS OF INTRANUCLEAR FLUORESCENCE DISTRIBUTION IN CONFOCAL MICROSCOPY, Computerized medical imaging and graphics, 17(3), 1993, pp. 189-200
Citations number
21
Categorie Soggetti
Radiology,Nuclear Medicine & Medical Imaging
Journal title
Computerized medical imaging and graphics → ACNP
ISSN journal
08956111
Volume
17
Issue
3
Year of publication
1993
Pages
189 - 200
Database
ISI
SICI code
0895-6111(1993)17:3<189:MF3VOI>2.0.ZU;2-U
Abstract
The introduction of the confocal laser scanning microscope makes it po ssible to acquire fluorescent specimens in 3D. We present basic image processing tools to enhance the data and to quantitate the morphology and topography of the intravolume elements. The tools were applied to the description of the spatial distribution of DNA replication sites i n mammalian cell nuclei as an example.