MODELING LIPOLYSIS IN A REVERSED MICELLAR SYSTEM .1. CONVENTIONAL BATCH REACTOR

Triglyceride hydrolysis using Chromobacterium viscosum lipase B in a r eversed micellar media was investigated in a batch-type reactor. The d ynamic evolution of the product concentration was analyzed using sever al mechanistic models, both from the literature and developed in this work. A kinetic model with nonlinear product inhibition was found to b e the most adequate for the description of batch hydrolysis data over an extensive range of time and substrate concentration. The obtained r ate equation described the time course of not only the reactions perfo rmed in this work, at different water contents (W0 = 7, 24) and pH val ues, but also the experimental results obtained in the literature with a Candida rugosa lipase. (C) 1993 John Wiley & Sons, Inc.