SURFACE-ENHANCED RAMAN-SPECTROMETRY OF AMILORIDE ON COLLOIDAL SILVER

Surface-enhanced Raman spectrometry (SERS) of the diuretic drug amilor ide is discussed. The SERS-active substrate used is colloidal silver, which is prepared at room temperature by simple tetrahydroborate reduc tion of an aqueous solution of silver nitrate. SERS detection of amilo ride in human urine is illustrated. The reproducibility of quantitativ e SERS data is supported by the simultaneous measurement of the scatte ring signal and transmitted light. Relative standard deviations at the 5% level are compatible with many practical analytical situations.