DIFFERENTIAL ANTAGONISM OF BREMAZOCINE-INDUCED AND U69,593-INDUCED ANTINOCICEPTION BY QUADAZOCINE - FURTHER FUNCTIONAL EVIDENCE OF OPIOD KAPPA-RECEPTOR MULTIPLICITY IN THE MOUSE
Pj. Horan et al., DIFFERENTIAL ANTAGONISM OF BREMAZOCINE-INDUCED AND U69,593-INDUCED ANTINOCICEPTION BY QUADAZOCINE - FURTHER FUNCTIONAL EVIDENCE OF OPIOD KAPPA-RECEPTOR MULTIPLICITY IN THE MOUSE, The Journal of pharmacology and experimental therapeutics, 266(2), 1993, pp. 926-933
In these studies, the antagonistic actions of methyl-2,6-methano-3-ben
zazocin-11-yl)-3-pentanone methanesulfonate (quadazocine) were evaluat
ed against the kappa-receptor-mediated antinociceptive effects of i.c.
v. rolidinylyl-oxaspiro(4,5)dec-8-yl)benzeneacetamide (U69,593) or bre
mazocine in the mouse warm water tail-flick test. Quadazocine produced
no antinociceptive effects alone, and it selectively antagonized the
actions of bremazocine, but not U69,593, in a dose- and time-related f
ashion, supporting previous suggestions of differences in kappa recept
ors mediating the antinociceptive effects of these agonists. Quadazoci
ne, however, also antagonized the antinociceptive effects of both DAMG
O (opioid mu agonist) and DPDPE (opioid delta agonist) at doses approx
imately 3-fold less than those needed to attenuate significantly the e
ffects of bremazocine. The structurally diverse kappa opioids lidinyl)
-cyclohexyl]benzo[b]-thiophene-4-acetamide (PD 117,302), ethylketocycl
azocine (EKC) and tifluadom were studied under kappa-selective conditi
ons, and the sensitivity of their effects to ro-N-[2(1-pyrrolidinyl)cy
clohexyl]benzeneacetamide [(-)-UPHIT] (kappa1 antagonist) or quadazoci
ne (kappa2 antagonist) was determined. On this basis PD 117,302, EKC a
nd tifluadom were classified as acting at opioid kappa1, kappa1, and k
appa2 receptors, respectively; EKC and tifluadom were also shown to ha
ve significant activity at opioid mu, but not delta, receptors. These
data demonstrating two-way differential antagonism of U69,593 and brem
azocine by quadazocine and (-)-UPHIT provide strong functional evidenc
e of opioid kappa receptor subtypes mediating supraspinal antinocicept
ion in the mouse. Additionally, the kappa-subtype classification of ka
ppa agonists of different structures begins to provide a basis for str
ucture-activity relationships of opioids acting at kappa1 and kappa2 r
eceptors.