CONSTRUCTION OF A BIOLUMINESCENT MYCOBACTERIUM AND ITS USE FOR ASSAY OF ANTIMYCOBACTERIAL AGENTS

Authors
ANDREW PW ROBERTS IS
Citation
Pw. Andrew et Is. Roberts, CONSTRUCTION OF A BIOLUMINESCENT MYCOBACTERIUM AND ITS USE FOR ASSAY OF ANTIMYCOBACTERIAL AGENTS, Journal of clinical microbiology, 31(9), 1993, pp. 2251-2254
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
31
Issue
9
Year of publication
1993
Pages
2251 - 2254
Database
ISI
SICI code
0095-1137(1993)31:9<2251:COABMA>2.0.ZU;2-L
Abstract
To show, as a model system, that mycobacteria can express heterologous luciferase genes and that bioluminescence can be a rapid method of me asuring antimycobacterial activity, a bioluminescent form of Mycobacte rium smegmatis was made by transformation with a Mycobacterium-Escheri chia coli shuttle vector containing the luxAB genes from Vibrio harvey i. The antimycobacterial effects of antibiotics and biocides could be assayed in real time by using bioluminescent M. smegmatis.