RAPID COMPETITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING A MONOCLONAL-ANTIBODY REACTING WITH A 15-KILODALTON TEGUMENTAL ANTIGEN OF SCHISTOSOMA-MANSONI FOR SERODIAGNOSIS OF SCHISTOSOMIASIS
Aj. Dasilva et al., RAPID COMPETITIVE ENZYME-LINKED-IMMUNOSORBENT-ASSAY USING A MONOCLONAL-ANTIBODY REACTING WITH A 15-KILODALTON TEGUMENTAL ANTIGEN OF SCHISTOSOMA-MANSONI FOR SERODIAGNOSIS OF SCHISTOSOMIASIS, Journal of clinical microbiology, 31(9), 1993, pp. 2315-2319
A competitive enzyme-linked immunosorbent assay (CELISA) for antibody
detection was developed by using a monoclonal antibody which reacts wi
th a 15-kDa tegumental antigen of the adult worm of Schistosoma manson
i. This monoclonal antibody was not able to react with antigens of Sch
istosoma japonicum or Schistosoma haematobium in enzyme-linked immunoe
lectrotransfer blot (EITB) and indirect immunofluorescence tests. The
assay was performed in a period of 1 h using an adult worm crude extra
ct antigen. To evaluate the CELISA, a total of 73 serum samples was an
alyzed: 35 were from S. mansoni-infected patients, 23 were from indivi
duals with parasitic infections other than schistosomiasis, and 14 wer
e from healthy individuals. All serum samples from healthy individuals
and from patients infected with other parasites were negative, as wer
e two (6%) samples from patients infected with S. mansoni. EITB analys
is showed that 32 of 33 CELISA-positive samples were positive in the E
ITB but with different patterns of reactivity. A 15-kDa protein reacte
d with 60% of serum samples, and a 60-kDa protein showed the highest l
evel of reactivity (85%). The two samples from patients infected with
S. mansoni that were negative in the CELISA reacted with 70-, 60-, 50-
, 47-, and 38-kDa proteins. One sample, positive in CELISA, did not re
act with proteins of the antigenic extract.