DIAGNOSIS OF TOXOPLASMA PARASITEMIA IN PATIENTS WITH AIDS BY GENE DETECTION AFTER AMPLIFICATION WITH POLYMERASE CHAIN-REACTION

We sought evidence of toxoplasma parasitemia among 37 people with acti ve or dormant Toxoplasma gondii infection or no evidence of infection. DNA was extracted from erythrocyte-free portions of blood samples, an d the T. gondii BI gene was amplified by the polymerase chain reaction . Evidence of T. gondii parasitemia was found in six patients with sev ere immunosuppression from AIDS and clinical evidence suggestive of or compatible with toxoplasmosis. Results were negative for patients unl ikely to have active toxoplasmosis. Gene detection after amplification with the polymerase chain reaction is a promising test for detection of parasitemia, and parasitemia should be tested for in patients with AIDS and unexplained fever or central nervous system abnormalities.