Ra. Dorrington et Tg. Cooper, THE DAL82 PROTEIN OF SACCHAROMYCES-CEREVISIAE BINDS TO THE DAL UPSTREAM INDUCTION SEQUENCE (UIS), Nucleic acids research, 21(16), 1993, pp. 3777-3784
Expression of the DAL2, DAL4, DAL7, DUR1,2, and DUR3 genes in S.cerevi
siae is induced by allophanate, the last intermediate in the allantoin
catabolic pathway. Analysis of the DAL7 promoter identified a dodecan
ucleotide, the DAL7 UIS, which was required for inducer-responsiveness
. Operation of the DAL 7 UIS required functional DAL81 and DAL82 gene
products. Since the DAL81 product was not an allantoin pathway-specifi
c regulatory factor, the DAL82 product was considered as the more like
ly candidate to be the DAL UIS binding protein. Using an E. coli expre
ssion system, we showed that DAL82 protein specifically bound to wild
type but not mutant DAL UIS sequences. DNA fragments containing DAL UI
S elements derived from various DAL gene promoters bound DAL82 protein
with different affinities which correlate with the degree of inducer-
responsiveness the genes displayed.