The response of activated sludge to starvation was studied in aerobic
conditions during a 10 days starvation period. The 30% sludge mass dec
rease was related to the degradation of both proteins and polysacchari
des as well as to the release of biopolymers in the water surrounding
the biomass. After 8 days, organic carbon release as a result of the s
ludge degradation was relatively low (almost-equal-to 2%) compared to
the respired carbon (42%). The decrease of the total number of bacteri
al cells followed the degradation of the solids only after 4 days. The
degradation of the sludge constituents occurred with a rapid decrease
of the respiratory activity. After 11 days the sludge was still able
to use an easily biodegradable substrate (yeast extract) contrary to t
he organic matter from primary wastewater. From our results, about 4 d
ays of storage of the sludges may represent a limit to be considered f
or a high mass reduction and a low biochemical activity loss (allowing
the recycling of a viable biomass).