ENZYMATIC PRODUCTION AND ISOLATION OF D-AMINO ACIDS FROM THE CORRESPONDING 5-SUBSTITUTED HYDANTOINS

Authors
DEEPA S SIVASANKAR B JAYARAMAN K PRABHAKARAN K GEORGE S PALANI P RAMESH KS SRINIVASAN CV KANDASAMY NR SADHUKHAN AK
Citation
S. Deepa et al., ENZYMATIC PRODUCTION AND ISOLATION OF D-AMINO ACIDS FROM THE CORRESPONDING 5-SUBSTITUTED HYDANTOINS, Process biochemistry, 28(7), 1993, pp. 447-452
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Process biochemistry → ACNP
ISSN journal
13595113
Volume
28
Issue
7
Year of publication
1993
Pages
447 - 452
Database
ISI
SICI code
1359-5113(1993)28:7<447:EPAIOD>2.0.ZU;2-M
Abstract
A detailed process for the production of D-amino acids starting from t he synthesis of 5-substituted hydantoin was established using whole ce lls of Agrobacterium radiobacter. About 94% substrate turnover was att ained at 30 mg/ml substrate concentration. On isolation and concentrat ion of the end product, 65% recovery was obtained. The product was fou nd to be 99% pure by high performance liquid chromatography (HPLC) and infrared (IR) analysis, and confirmed by optical rotation.