INCORPORATION OF O-18-LABELED WATER INTO OXYGENATED PRODUCTS PRODUCEDBY THE ENZYME DEACETOXY DEACETYLCEPHALOSPORIN C-SYNTHASE

Authors
BALDWIN JE ADLINGTON RM CROUCH NP PEREIRA IAC
Citation
Je. Baldwin et al., INCORPORATION OF O-18-LABELED WATER INTO OXYGENATED PRODUCTS PRODUCEDBY THE ENZYME DEACETOXY DEACETYLCEPHALOSPORIN C-SYNTHASE, Tetrahedron, 49(34), 1993, pp. 7499-7518
Citations number
26
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron → ACNP
ISSN journal
00404020
Volume
49
Issue
34
Year of publication
1993
Pages
7499 - 7518
Database
ISI
SICI code
0040-4020(1993)49:34<7499:IOOWIO>2.0.ZU;2-5
Abstract
O-18-labelling experiments have been conducted with the enzyme deaceto xy/deacetylcephalosporin C synthase. Incubations of [2-C-13,3-H-2]peni cillin N and [4-H-2]exomethylene cephalosporin C were carried out with O-18(2) or (H2O)-O-18, and O-18-incorporation from both label sources was observed into the oxygenated enzymic products. This is believed t o be the first example of (H2O)-incorporation into products of an alph a-ketoglutarate dependent dioxygenase. An oxygen-exchange process at a n enzyme-bound intermediate is proposed to account for this incorporat ion.