EFFECTS OF SURGERY ON THE GENERATION OF LYMPHOKINE-ACTIVATED KILLER-CELLS IN PATIENTS WITH BREAST-CANCER

Natural killer (NK) cell activity and the capacity to generate lymphok ine-activated killer (LAK) cell activity were studied in 43 patients w ith operable breast cancer before and after surgery. Results were comp ared with those from ten healthy subjects. Patients with breast cancer had normal LAK and NK cell activity before surgery. A subgroup of pat ients with stage III disease had depressed LAK cell activity (P < 0.01 3). NK cell activity decreased by over 50 per cent on the first day af ter surgery and did not return to preoperative levels by day 7 (P < 0. 0005). Generation of LAK cell activity was unaffected by surgery. The addition of 10 per cent autologous plasma to the culture medium during the induction of LAK cell activity in vitro did not suppress LAK cell activity in patients with breast cancer. These results suggest that p erioperative adjuvant immunotherapy based on interleukin 2 and LAK cel ls is not ruled out by systemic suppressive effects from either cancer or surgery.