The hydrolysis of the amylopectin of gelatinized waxy barley starch by
Bacillus licheniformis alpha-amylase occurred in a non-random way, sh
owing accumulation of smaller-size hydrolysis products. The hydrolysis
of barley starch by Bacillus acidopullulyticus pullulanase also began
with a rapid decrease in the molecular size of amylopectin, indicatin
g that the enzyme was capable of hydrolyzing the alpha-1,6-linkages in
the middle of the amylopectin molecule.