N. Faisant et al., STRUCTURAL DISCREPANCIES IN RESISTANT STARCH OBTAINED IN-VIVO IN HUMANS AND IN-VITRO, Carbohydrate polymers, 21(2-3), 1993, pp. 205-209
In-vivo resistant starch (RS) was collected at the end of the human il
eum by an intubation technique after ingestion of two partly resistant
starches (retrograded (R) or complexed (C) high amylose maize starch)
. In-vitro RS fractions were prepared enzymatically from R and C accor
ding to three methods of RS determination in food. Physicochemical cha
racteristics of these two different fractions were compared. The ileal
RS appeared to consist of three fractions: a first population of high
molecular weight alpha-glucans attributed to amorphous potentially di
gestible material, a second made of B-type retrograded amylose crystal
lites and a third containing oligosaccharides. The in-vitro RS fractio
ns showed no high molecular weight molecules, due to more extensive hy
drolysis in the in-vitro procedure. Therefore, none of the in-vitro RS
determinations allowed the isolation of a fraction qualitatively simi
lar to in-vivo RS.