IDENTIFICATION OF CYCLIC-NUCLEOTIDE DERIVATIVES SYNTHESIZED FOR RADIOIMMUNOASSAY DEVELOPMENT BY FAST-ATOM-BOMBARDMENT WITH COLLISION-INDUCED DISSOCIATION AND MASS-ANALYZED ION KINETIC-ENERGY SPECTROSCOPY

The syntheses of 2'-O-succinyl, 2'-O-succinyltyrosinyl methyl ester an d 2'-O-succinyliodotyrosinyl methyl ester derivatives of a cyclic nucl eotide, derivatives necessary for the successful development of a spec ific radioimmunoassay, are described. Fast atom bombardment with colli sion-induced dissociation and mass-analysed ion kinetic energy spectro scopy were used to verify the positions of substitution and the retent ion of the 3',5'-cyclic phosphate moiety. Comparison of spectra produc ed after different iodination times permitted the optimization of the reaction conditions.