SOMATOSTATIN INHIBITS PEPSINOGEN SECRETION WITHOUT INFLUENCING CYTOSOLIC-FREE CA2-OCTAPEPTIDE IN RAT CHIEF CELLS( INCREASE INDUCED BY CARBACHOL AND CHOLECYSTOKININ)
T. Tanaka et al., SOMATOSTATIN INHIBITS PEPSINOGEN SECRETION WITHOUT INFLUENCING CYTOSOLIC-FREE CA2-OCTAPEPTIDE IN RAT CHIEF CELLS( INCREASE INDUCED BY CARBACHOL AND CHOLECYSTOKININ), Biological & pharmaceutical bulletin, 16(8), 1993, pp. 767-770
Gastric chief cells were isolated from the rat stomach in an attempt t
o identify those involved in the mechanism of action of somatostatin o
n pepsinogen secretion. The effects of several kinds of secretagogues
on cytosolic free Ca2+ concentration ([Ca2+]i) were examined in the ra
t chief cells. Carbachol and cholecystokinin octapeptide (CCK-8) marke
dly induced [Ca2+]i increase, while histamine, gastrin I and secretin
did not. Carbachol and CCK-8 also stimulated pepsinogen secretion. A s
imilar dose-response relationship was seen in carbachol- and CCK-8-ind
uced [Ca2+]i increase and pepsinogen secretion. Somatostatin did not i
nhibit carbachol- or CCK-8-induced [Ca2+]i increase, but did inhibit c
arbachol- and CCK-8-induced pepsinogen secretion by 30 and 50%, respec
tively.