PRODUCTION AND CHARACTERIZATION OF MOUSE MONOCLONAL-ANTIBODIES DIRECTED AGAINST CANINE IGE AND IGG

Immediate-type hypersensitivity reactions have been studied infrequent ly in dogs, in part because of limited availability of antisera specif ic for canine IgE. A series of murine hybridoma cell lines were prepar ed, that produced monoclonal antibodies (MAb) with specificity for can ine immunoglobulin E (IgE) and IgG. The MAb were tested for their abil ity to induce a reverse cutaneous anaphylaxis reaction in dog skin, to neutralize the Prausnitz-Kustner reactivity of atopic dog serum, to s erve as a ligand in immunoaffinity chromatography, and to bind to IgE and other Ig subclasses in several ELISA systems. Some of the MAb prod uced were found to be specific for canine IgE. Other MAb recognized co mmon or similar determinants on IgE and IgG, or on IgG and IgM, though with apparently differing affinities. Heat or acid treatment of canin e IgE abolished most, but not all, of the reactivity with the anti-IgE MAb. These MAb will be useful for further study of IgE-mediated pheno mena in the dog.