Gf. Wiegertjes et al., ESTIMATION OF THE GENETIC-VARIATION IN COMPLEMENT ACTIVITY OF COMMON CARP (CYPRINUS-CARPIO L), Veterinary immunology and immunopathology, 37(3-4), 1993, pp. 309-319
The complement status of hybrid, laboratory raised carp was determined
by an in vitro approach of the alternate complement activity (ACH50)
and total haemolytic activity (CH50), and by measurement of serum C3 l
evels. The lysis of target sheep red blood cells (RBC) in the haemolyt
ic assay for CH50 activity depended, amongst others, on the haemolysin
concentration in the assay. Rocket electrophoresis showed a mean seru
m C3 concentration of 0.95 mg ml-1. The variation for both ACH50 and C
H50 haemolytic activity was approximately 30%. The degree of genetic d
etermination of the parameters was investigated by estimation of their
repeatabilities, which were relatively high for CH50 (0.71) and ACH50
activity (0.72), but lower for C3 levels (0.54). Correlations between
ACH50 values and C3 levels were significant, but moderate (0.54-0.58)
.