OPTIMIZED CAPILLARY ZONE ELECTROPHORETIC SEPARATION OF CHLORINATED PHENOLS

A mixture of thirteen chlorinated phenols was resolved within 16 min b y using a 57 cm x 75 mu m I. D. fused-silica tube with a 50 mM sodium phosphate buffer (pH 6.9) electrolyte, under an 18 kV potential. The e lectrophoretic behaviour of chlorophenol congeners was investigated in order to optimize their separation as a function of the running buffe r pH, concentration and applied voltage. A migration order depending o n both charge and size of the solutes was established. Selectivity is strongly affected by the electrolyte concentration, in a manner that c annot be easily predicted. Quantitative aspects of capillary zone elec trophoresis are also discussed.