I. Rodriguezmoldes et al., DISTRIBUTION OF SUBSTANCE P-LIKE IMMUNOREACTIVITY IN THE BRAIN OF THEELASMOBRANCH SCYLIORHINUS-CANICULA, Journal of comparative neurology, 335(2), 1993, pp. 228-244
Immunohistochemical methods were used to study the distribution of sub
stance P in the brain of the small-spotted dogfish (Scyliorhinus canic
ula). Substance P-like immunoreactive (SP-IR) cell bodies and fibers w
ere widely distributed. In the telencephalon, sparse populations of SP
-IR neurons are present in the olfactory bulbs, pallium, and subpalliu
m. In the subpallium numerous SP-IR boutons form unusual coats (''peri
cellular appositions'') on SP-immunonegative neurons. In the diencepha
lon numerous SP-IR cerebrospinal fluid-contacting neurons are present
in the preoptic recess organ and organon vasculosum hypothalami. Numer
ous SP-IR fibers also run in the hypothalamus, although no immunoreact
ivity was observed in the habenulo-interpeduncular system. A terminal
field of SP-IR fibers is present in the median eminence. In the mesenc
ephalic tegmentum, SP-IR neurons were observed in the Edinger-Westphal
nucleus. SP-IR fibers are present at high density in the basal tegmen
tum, forming a conspicuous tract. In the hindbrain, numerous SP-IR fib
ers were observed in the isthmal region, the trigeminal descending roo
t, the visceral sensory area and commissural nucleus, and the viscerom
otor column. SP-IR fibers occur at high density in the substantia gela
tinosa of the rostral spinal cord. (C) 1993 Wiley-Liss, Inc.