EFFECT OF OZONE ON METABOLIC-ACTIVITIES OF RAT HEPATOCYTES AND MOUSE PERITONEAL MACROPHAGE

Peritoneal macrophage from mice and isolated hepatocytes from rats wer e exposed to ozone. Ozone dosages were expressed as 0-5 nmol/10(6) cel ls. Measurements were made of viability, glucose transport, glutathion e, glyceraldehyde-3-phosphate dehydrogenase, Mg-ATPase, Na/K-ATPase, a nd lipid synthesis. The most sensitive parameter was glyceraldehyde-3- phosphate dehydrogenase in the peritoneal macrophage. In hepatocytes b oth lipid synthesis and glyceraldehyde-3-phosphate dehydrogenase were sensitive to ozone. Effects on viability, glucose transport, Mg-ATPase , and Na/K-ATPase were small to negligible in both cell types.