IDENTIFICATION OF THE PUTATIVE HEXOSE-PHOSPHATE TRANSLOCATOR OF AMYLOPLASTS FROM CAULIFLOWER BUDS

Authors
BATZ O SCHEIBE R NEUHAUS HE
Citation
O. Batz et al., IDENTIFICATION OF THE PUTATIVE HEXOSE-PHOSPHATE TRANSLOCATOR OF AMYLOPLASTS FROM CAULIFLOWER BUDS, Biochemical journal, 294, 1993, pp. 15-17
Citations number
16
Categorie Soggetti
Biology
Journal title
Biochemical journal → ACNP
ISSN journal
02646021
Volume
294
Year of publication
1993
Part
1
Pages
15 - 17
Database
ISI
SICI code
0264-6021(1993)294:<15:IOTPHT>2.0.ZU;2-J
Abstract
Starch synthesis in amyloplasts isolated from cauliflower buds is stro ngly inhibited by the addition of micromolar concentrations of 4,4'-di -isothiocyano-2,2'-stilbenedisulphonic acid (DIDS). Using [H-3]DIDS it was possible to label specifically a 31.6 kDa membrane protein of the envelope fraction of isolated amyloplasts. The intensity of the radio active label was decreased in the presence of glucose 6-phosphate or d ihydroxyacetone phosphate, indicating that this protein might be the a myloplastic hexose-phosphate translocator.