PREPARATION OF CHROMOSOME-SPECIFIC PAINTS AND COMPLETE ASSIGNMENT OF CHROMOSOMES IN THE PIG FLOW KARYOTYPE

Citation
Cf. Langford et al., PREPARATION OF CHROMOSOME-SPECIFIC PAINTS AND COMPLETE ASSIGNMENT OF CHROMOSOMES IN THE PIG FLOW KARYOTYPE, Animal genetics, 24(4), 1993, pp. 261-267
Citations number
23
Categorie Soggetti
Genetics & Heredity","Veterinary Sciences
Journal title
ISSN journal
02689146
Volume
24
Issue
4
Year of publication
1993
Pages
261 - 267
Database
ISI
SICI code
0268-9146(1993)24:4<261:POCPAC>2.0.ZU;2-5
Abstract
Sorted chromosomes from each of the 20 clusters of the male porcine bi variate flow karyotype were amplified and biotinylated using DOP-PCR. The chromosomes comprising each cluster were identified by fluorescenc e in situ hybridization (FISH) of the 20 probes to R-banded male pig m etaphase spreads. A standard flow karyotype for the pig is presented.