DETERMINATION OF PHENOL IN WASTES AND WATER USING AN ENZYME SENSOR

Biosensors for the determination of phenol in real matrices such as oi l press and industrial wastes and sea-, lake and river waters are prop osed. Optimization of the experimental parameters, especially regardin g enzyme immobilization, was performed. The biosensors are based on a Clark oxygen electrode coupled with tyrosinase immobilized by three di fferent methods. Biosensors were also tested in order to verify whethe r their direct use with environmental samples and in in situ analysis is possible.