N-phosphorylhistidine was converted into its ester and phosphoric este
r exchanged products in alcoholic media. It was demonstrated that the
co-participation of the three functional groups of phosphoryl, imidazo
lyl, and carboxyl in the molecule is essential for these reactions to
occur. Also, the neat phosphoryl transfer reaction occurs with N(alpha
), N(Im)-Bis-phosphohistidine.