Ep. Brandon et al., CULTURED HUMAN ENDOTHELIAL-CELLS EXPRESS THE 140-KD ISOFORM OF NEURALCELL-ADHESION MOLECULE (NCAM), International journal of immunopathology and pharmacology, 6(2), 1993, pp. 77-84
Cultured human saphenous vein endothelial cells (HSVEC) were shown to
express neural cell adhesion molecule (NCAM) by cytofluorographic anal
ysis. Radioimmunoprecipitation and subsequent electrophoresis of sampl
es prepared from S-35-methionine/cysteine metabolically labeled HSVEC
yielded a 140 kD band, consistent with one of the known NCAM isoforms.
Northern blot analysis of HSVEC revealed a single species of NCAM- en
coding mRNA of approximately 6.1 kb. Together, these data indicate tha
t cultured endothelial cells produce and express on their surface the
140 kD isoform of NCAM.