OXIDATION OF [U-C-14]ACETATE BY THE SHEEP CONCEPTUS BETWEEN DAYS 13 AND 19 OF PREGNANCY

Acetate metabolism by the sheep conceptus was assessed by measuring CO 2 production during a 2.5-h incubation of embryos and samples of the e xtraembryonic membranes in HEPES-bufferd media containing 1.12 mM [U-C -14]acetate. The rate of oxidation of acetate by embryonic tissue show ed little change between Days 13 and 15 of pregnancy but greatly decre ased by Days 17 and 19. By contrast, oxidation of the substrate by the trophoblast increased substantially with development and was rive tim es the early rate by Day 19. Oxidation of acetate by the yolk sac also increased 4-fold between Days 17 and 19. The addition of glucose to i ncubations of extraembryonic membranes resulted in some reduction in t he oxidation of acetate by the yolk sac and allantois but had little e ffect on the trophoblast. At Days 13 and 15, the rate of oxidation of acetate by the embryonic disc was 6-7 times that by the trophoblast. A s development progressed, this situation was reversed and by Day 19 th e trophoblast metabolized more than five times the amount of acetate p er mug than did the Day-19 embryo. Although acetate metabolism by yolk sac and allantois on Day 17 was low, its metabolism by the yolk sac i ncreased to values similar to those for the trophoblast at Day 19 but its utilization by the allantoic membrane remained low. Comparison of the estimates of ATP generated from acetate by these tissue with those published for glucose demonstrates that acetate is much less effectiv e than glucose for the provision of metabolic energy.