Vn. Uversky et al., EFFECT OF SINGLE AMINO-ACID REPLACEMENT O N T4 PHAGE LYSOZYME STABILITY .1. POINT MUTATION ASN101-]ASP, Biofizika, 38(4), 1993, pp. 602-605
The amino acid replacement Asn101-->Asp in the T4 phage lysozyme was o
btained by site-directed mutagenesis and the plasmid mutant protein ex
pression was constructed. Though the mutant protein circular dichroism
(CD) spectrum is virtually unchanged as compared with the wild type p
rotein and the enzymatic activity is 90% of that of the wild type prot
ein, the stability of this mutant to urea-induced unfolding decreasees
and two stages in the denaturation process are observed.