M. Allaith et Fl. Pearce, ON THE MECHANISM OF ACTION OF SODIUM ORTHOVANADATE IN INDUCING HISTAMINE-RELEASE FROM RAT PERITONEAL MAST-CELLS, International archives of allergy and immunology, 101(4), 1993, pp. 385-391
Sodium orthovanadate produced a dose-dependent release of histamine an
d prostaglandin D2 from rat peritoneal mast cells. The release of hist
amine was selectively inhibited by the anion channel blockers SITS and
DIDS, and by theophylline and dibutyryl cyclic-AMP, but was unaffecte
d by disodium cromoglycate and lanthanum ions. Unlike IgE-directed lig
ands, vanadate did not produce any change in the intracellular concent
ration of cyclic-AMP but did promote a substantial uptake of calcium-4
5 from the incubation medium. This effect paralleled the release of hi
stamine. These results are discussed in terms of the possible mode of
action of vanadate.