POTENTIOMETRIC MONITORING OF PROTEINS .6. INDIRECT POTENTIOMETRY

In a study of the indirect potentiometry of amino acids and proteins w ith metal ions it has been discovered that electrode potential respons es to organic ligand concentrations can be linear over a range of tens of millivolts. In this paper the reasons for this are considered and a general model is presented in terms of an electrode of the second ki nd. Experimental results for the indirect potentiometry of a number of amino acids and proteins are then presented and are discussed in the context of the model derived. Applications to flow injection analysis and to the determination and identification of proteins eluting from a gel chromatography column are also considered.