PLANT-REGENERATION FROM NUCELLAR TISSUES OF AEGLE-MARMELOS THROUGH ORGANOGENESIS

Authors
HOSSAIN M KARIM MR ISLAM R JOARDER OI
Citation
M. Hossain et al., PLANT-REGENERATION FROM NUCELLAR TISSUES OF AEGLE-MARMELOS THROUGH ORGANOGENESIS, Plant cell, tissue and organ culture, 34(2), 1993, pp. 199-203
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
Plant cell, tissue and organ culture → ACNP
ISSN journal
01676857
Volume
34
Issue
2
Year of publication
1993
Pages
199 - 203
Database
ISI
SICI code
0167-6857(1993)34:2<199:PFNTOA>2.0.ZU;2-P
Abstract
A protocol for organogenesis from nucellar explants excised from ferti lized ovules of immature fruits of Aegle marmelos Corr. was developed. Adventitious buds were initiated on Murashige and Skoog's (MS) medium containing various combinations of 6-benzyladenine (BA), alpha-naphth alene-acetic acid (NAA), 3-indoleacetic acid and gibberellic acid. Med ium containing 4.4 mum BA and 2.7 muM NAA produced the maximum number of adventitious buds per explant. Shoots were elongated by transferrin g explants with shoot buds to medium with a low concentration of BA (0 .44 muM). Rooting of in vitro-regenerated shoots was obtained in half- strength MS medium with 4.9 muM indole-3-butyric acid. This is the fir st report of plant regeneration from nucellar explants of A. marmelos.