G. Demarch et al., POTENTIAL OF SOMATIC EMBRYOGENESIS IN PRUNUS-AVIUM IMMATURE ZYGOTIC EMBRYOS, Plant cell, tissue and organ culture, 34(2), 1993, pp. 209-215
For the purpose of developing somatic embryogenesis in Prunus avium L.
, immature zygotic embryos, collected from five donor trees and sorted
into two size classes (C1: 2.5-3.5 and C2: 3.6-4.5 mm), received vari
ous experimental treatments. When cultured for 10 days on an inductive
medium containing 18.1 muM 2,4-dichlorophenoxyacetic acid (2,4-D) and
9.3 muM kinetin, then transferred to fresh medium without growth regu
lators, 2.5% of the Cl class cotyledons expressed direct somatic embry
ogenesis. C2 class cotyledons were less responsive. The response was a
lso influenced by the chosen donor tree. In a few cases, spontaneous g
ermination occurred. The presence of a root meristem was clearly demon
strated by histological examination of longitudinal sections. The repl
acement of half the amount of 2,4-D, present in the inductive medium m
entioned above, by the same quantity of naphthaleneacetic acid reduced
the incidence of somatic embryogenesis. Conversely, a rhizogenic resp
onse was strongly enhanced. When submitted to an inductive medium cont
aining indoleacetic acid and zeatin without any subcultures for 3 mont
hs, C1 class cotyledons were the most morphogenic and developed leaves
and cotyledon-like structures.