FORMATION AND GROWTH OF MULTICELLULAR SPHEROIDS IN MEDIA CONTAINING LOW CONCENTRATIONS OF AGAROSE

Authors
KUWASHIMA Y YAMADA T SAIO M TAKAMI T
Citation
Y. Kuwashima et al., FORMATION AND GROWTH OF MULTICELLULAR SPHEROIDS IN MEDIA CONTAINING LOW CONCENTRATIONS OF AGAROSE, Cancer letters, 71(1-3), 1993, pp. 31-33
Citations number
11
Categorie Soggetti
Oncology
Journal title
Cancer letters → ACNP
ISSN journal
03043835
Volume
71
Issue
1-3
Year of publication
1993
Pages
31 - 33
Database
ISI
SICI code
0304-3835(1993)71:1-3<31:FAGOMS>2.0.ZU;2-W
Abstract
Human melanoma HMV-1 cells formed efficiently multicellular spheroids in media containing low concentrations (0.001-0.01%) of agarose (LCAM) . In addition, the spheroids were prevented from deformation without a pparent delay of the growth rate. On the other hand, spheroid formatio n was inhibited markedly by LCAM in the case of murine melanoma B16 ce lls. Also, LCAM could not prevent the B16 spheroids from deformation. These results indicate that LCAM acts differently depending on the cel l types used for spheroid formation.