Wj. Jakubas et al., RUFFED GROUSE TOLERANCE AND BIOTRANSFORMATION OF THE PLANT SECONDARY METABOLITE CONIFERYL BENZOATE, The Condor, 95(3), 1993, pp. 625-640
Ruffed Grouse (Bonasa umbellus) consumption of quaking aspen (Populus
tremuloides) flower buds is determined, in part, by the concentration
of coniferyl benzoate (CB) in the bud. Understanding the physiological
effects of this compound may clarify whether the association between
annual fluctuations in CB and Ruffed Grouse population levels has a ca
use/effect relationship. We determined the maximum level of dietary CB
that Ruffed Grouse can tolerate by feeding captive grouse a formulate
d diet treated with eight concentrations of CB. Loss of body mass was
used to indicate toxicity. Biotransformation products from CB were ide
ntified in order to determine the detoxication mechanisms employed and
the presence of potentially toxic CB metabolites. Ruffed Grouse will
consume up to 2 g kg-1 day-1 of CB before rapid mass loss ensues. High
dietary levels of CB were associated with decreased food intake and i
ncreased water excretion. Serum uric acid, aspartate aminotransferase,
and alanine aminotransferase levels did not change significantly with
increased levels of dietary CB. Mass loss that was associated with a
CB intake of 2.3 g kg-1 day-1 could be explained by decreased food int
ake but could not be attributed to any specific toxic effect. It is un
certain whether the decreased food consumption that occurred at high C
B intake levels was prompted by toxicity avoidance or decreased food p
alatability. Ruffed Grouse appear to use glucuronic acid, sulfate, and
ornithine conjugation, along with hydrolysis, reduction, and oxygenat
ion reactions, when detoxifying CB. Nine biotransformation products we
re identified, including ferulic acid and 4-vinylguaiacol. The latter
compounds are known to interfere with reproduction in other animals.