Mk. Zubko et al., SELECTION OF ANTHOCYANIN-ACCUMULATING POTATO (SOLANUM-TUBEROSUM L) CELL-LINES FROM CALLI DERIVED FROM SEEDLINGS PRODUCED BY GAMMA-IRRADIATED SEEDS, Plant cell reports, 12(10), 1993, pp. 555-558
Callus cell lines of potato (Solanum tuberosum L. cv. Zarevo) were obt
ained from seedlings germinated from gamma-irradiated seeds (200 Gy).
Some of these cell lines produce red-violet pigments which were identi
fied as acylated anthocyanins. The major anthocyanin was determined to
be peonidin 3-O-[6-O-(4-0-E-p-coumaroyl-rhamnosyl)-glucoside (''peona
nin''). Single cell-derived protoclones from non-pigmented protoplasts
sometimes also gave rise to pigmented cell clusters thus indicating t
hat the changes in the expression of the anthocyanin pathway can also
occur after the stage of initial callus induction.