This study was aimed at investigating the demineralizing conditions of
milk casein hydrolysates prepared enzymatically by electrodialysis (E
D). The changes in ash, protein, free amino acid content and so on bef
ore and after ED treatment were investigated. All ED treatments were c
arried out after Low Rejection Reverse Osmosis Membrane (LRO) treatmen
t. (1) The demineralization of casein hydrolysates proceeded effective
ly as the operating temperature rose. (2) The relationship between ash
content and electric conductivity was expressed as a linear line. (3)
The amount of ash and free amino acid decreased with ED treatment. In
treatment with recovery rates of 87.8, 78.4, and 81.4%, casein hydrol
ysates with deashing rates of 72.5, 75.7 and 78.6% were obtained, resp
ectively. (4) It was found that calcium was demineralized more easily
than the other minerals in casein hydrolysates. On the other hand, it
was difficult to deash sodium. (5) Both glutamic acid and aspartic aci
d showed remarkable decreases. Arginine also showed a tendency to decr
ease. The other amino acids, however, didn't decrease and, on the cont
rary, showed a tendency to become more concentrated. (6) ED treatment
was proved to be as effective as LRO treatment in improving the taste
of casein hydrolysates caused by free amino acids.