AN INTEGRATED METABOLIC MODELING APPROACH TO DESCRIBE THE ENERGY EFFICIENCY OF ESCHERICHIA-COLI FERMENTATIONS UNDER OXYGEN-LIMITED CONDITIONS - CELLULAR ENERGETICS, CARBON FLUX, AND ACETATE PRODUCTION
Yf. Ko et al., AN INTEGRATED METABOLIC MODELING APPROACH TO DESCRIBE THE ENERGY EFFICIENCY OF ESCHERICHIA-COLI FERMENTATIONS UNDER OXYGEN-LIMITED CONDITIONS - CELLULAR ENERGETICS, CARBON FLUX, AND ACETATE PRODUCTION, Biotechnology and bioengineering, 42(7), 1993, pp. 843-853
An integrated metabolic model for the production of acetate by growing
Escherichia coli on glucose under serobic conditions is presented. Th
e model is based on parameters which are easily determined by experime
nts. Forming the basis for this integrated metabolic model are the 12
principal precursor metabolites for biosynthetic pathways, the Embden-
Meyerhof-Parnas pathway, the pentose phosphate cycle, the tricarboxyli
c acid cycle and the anapleurotic reactions, the Crabtree effect, the
Pasteur effect, and the details of bacterial respiration. The result c
an be used to explain phenomena often observed in industrial fermentat
ions, i.e., increased acetate production which follows from high gluco
se uptake rate, a low oxygen concentration, a high specific growth rat
e, or a combination of these conditions. (C) 1993 John Wiley & Sons, I
nc.