The use of protein electrophoretic data for determining species bounda
ries in amphipods is addressed. Analysis of published literature on ge
netic differentiation in amphipods showed that pairs of allopatric pop
ulations which have genetic identities (I) above a value of 0.85 proba
bly represent intraspecific populations, whereas pairs of populations
which have genetic identities below about 0.45 probably represent diff
erent species. It was recommended that if I values fall between 0.45 a
nd 0.85, additional factors such as evidence of a lack of gene flow be
tween the populations, and concordant morphological variation should b
e considered.