PURIFICATION AND PARTIAL CHARACTERIZATION OF A MITOGENIC LECTIN FROM THE LATEX OF EUPHORBIA-MARGINATA

Authors
STIRPE F LICASTRO F MORINI MC PARENTE A SAVINO G ABBONDANZA A BOLOGNESI A FALASCA AI ROSSI CA
Citation
F. Stirpe et al., PURIFICATION AND PARTIAL CHARACTERIZATION OF A MITOGENIC LECTIN FROM THE LATEX OF EUPHORBIA-MARGINATA, Biochimica et biophysica acta, 1158(1), 1993, pp. 33-39
Citations number
24
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1158
Issue
1
Year of publication
1993
Pages
33 - 39
Database
ISI
SICI code
0006-3002(1993)1158:1<33:PAPCOA>2.0.ZU;2-A
Abstract
A lectin was purified from the latex of Euphorbia marginata by affinit y chromatography on acid-treated Sepharose 6B and elution with lactose . The lectin is a glycoprotein composed of two identical subunits with M(r) 30 000, approx. The haemagglutinating activity of the lectin is not specific for any human blood group, and is inhibited by galactose and galactose-containing sugars and by gentiobiose. The lectin is stro ngly mitogenic for human T-lymphocytes and induces the release of inte rleukin-1beta and tumor necrosis factor-alpha from cultured mononuclea r cells.