CONTINUOUS PROTEIN-SYNTHESIS SYSTEM WITH ESCHERICHIA-COLI S30 EXTRACTCONTAINING ENDOGENOUS-T7 RNA-POLYMERASE

A modified continuous-flow, cell-free (CFCF), coupled transcription an d translation system was constructed using an Escherichia coli S30 ext ract containing endogenous T7 RNA polymerase (S30 extract-T7pol), whic h was prepared from IPTG induced E. coli BL21(DE3) cells. Though the p lasmid pT7CT, which harbored both a bla gene and a chloramphenicol ace tyl transferase (CAT) encoding gene under the control of a T7 promoter , was used as a template, only CAT protein was continuously produced b y this CFCF-coupled system.