CONSTRUCTION OF A NEW SHUTTLE VECTOR PSTE33 AND ITS STABILITIES IN BACILLUS-STEAROTHERMOPHILUS, BACILLUS-SUBTILIS, AND ESCHERICHIA-COLI

Authors
NARUMI I NAKAYAMA N NAKAMOTO S KIMURA T YANAGISAWA T KIHARA H
Citation
I. Narumi et al., CONSTRUCTION OF A NEW SHUTTLE VECTOR PSTE33 AND ITS STABILITIES IN BACILLUS-STEAROTHERMOPHILUS, BACILLUS-SUBTILIS, AND ESCHERICHIA-COLI, Biotechnology letters, 15(8), 1993, pp. 815-820
Citations number
27
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
15
Issue
8
Year of publication
1993
Pages
815 - 820
Database
ISI
SICI code
0141-5492(1993)15:8<815:COANSV>2.0.ZU;2-F
Abstract
A shuttle vector that could replicate in B. stearothermophilus, B. sub tilis, and E. coli was constructed from B. stearothermophilus cryptic plasmid pSTK1, E. coli vector pUC19, and a thermostable kanamycin-resi stance marker. This new vector was stably maintained in B. stearotherm ophilus at 67-degrees-C without selective pressure.