SYNTHESIS OF OLIGODEOXYNUCLEOTIDES ELIMINATING THE USE OF ACETONITRILE

Authors
SMRT J ARNOLD L SVOBODA J HAK R ROSENBERG I
Citation
J. Smrt et al., SYNTHESIS OF OLIGODEOXYNUCLEOTIDES ELIMINATING THE USE OF ACETONITRILE, Collection of Czechoslovak Chemical Communications, 58(7), 1993, pp. 1692-1698
Citations number
17
Categorie Soggetti
Chemistry
Journal title
Collection of Czechoslovak Chemical Communications → ACNP
ISSN journal
00100765
Volume
58
Issue
7
Year of publication
1993
Pages
1692 - 1698
Database
ISI
SICI code
0010-0765(1993)58:7<1692:SOOETU>2.0.ZU;2-A
Abstract
H-Phosphonate synthesis of oligodeoxynucleotides is described in which acetonitrile is replaced by dichloromethane. This solvent is thereaft er easily regenerated and reused. Practically ''one solvent'' procedur e of oligonucleotide synthesis has been established. The preparation o f -N2-(N,N-dimethylaminomethylene)-2'-deoxyguanosine (Ic) and -N6-(N,N -dimethylaminomethylene)-2'-deoxyadenosine (Id) is described.