USE OF BIOCHEMICAL-GENETIC MARKERS TO DISCRIMINATE BETWEEN ADDUCTOR MUSCLES OF THE SEA SCALLOP (PLACOPECTEN-MAGELLANICUS) AND THE ICELAND SCALLOP (CHLAMYS-ISLANDICA)

Citation
E. Kenchington et al., USE OF BIOCHEMICAL-GENETIC MARKERS TO DISCRIMINATE BETWEEN ADDUCTOR MUSCLES OF THE SEA SCALLOP (PLACOPECTEN-MAGELLANICUS) AND THE ICELAND SCALLOP (CHLAMYS-ISLANDICA), Canadian journal of fisheries and aquatic sciences, 50(6), 1993, pp. 1222-1228
Citations number
30
Categorie Soggetti
Marine & Freshwater Biology",Fisheries
ISSN journal
0706652X
Volume
50
Issue
6
Year of publication
1993
Pages
1222 - 1228
Database
ISI
SICI code
0706-652X(1993)50:6<1222:UOBMTD>2.0.ZU;2-A
Abstract
Three biochemical techniques were applied to adductor muscles of Placo pecten magellanicus and Chlamys islandica, two commercially important scallops, to search for species-specific genetic markers. Allozyme ele ctrophoresis identified two enzyme systems, mannose phosphate isomeras e and glutamic oxaloacetate transaminase, which appear to be diagnosti c. Sequencing of the 18S rRNA gene in each species showed 15 nucleotid e differences over 1815 base pairs. Digestion of the gene with the res triction enzyme XHO I released two fragments in Placopecten and three in Chlamys. All three techniques could be developed for management pur poses as part of an enforcement programme to identify illegally caught scallops.