USE OF BIOCHEMICAL-GENETIC MARKERS TO DISCRIMINATE BETWEEN ADDUCTOR MUSCLES OF THE SEA SCALLOP (PLACOPECTEN-MAGELLANICUS) AND THE ICELAND SCALLOP (CHLAMYS-ISLANDICA)
E. Kenchington et al., USE OF BIOCHEMICAL-GENETIC MARKERS TO DISCRIMINATE BETWEEN ADDUCTOR MUSCLES OF THE SEA SCALLOP (PLACOPECTEN-MAGELLANICUS) AND THE ICELAND SCALLOP (CHLAMYS-ISLANDICA), Canadian journal of fisheries and aquatic sciences, 50(6), 1993, pp. 1222-1228
Three biochemical techniques were applied to adductor muscles of Placo
pecten magellanicus and Chlamys islandica, two commercially important
scallops, to search for species-specific genetic markers. Allozyme ele
ctrophoresis identified two enzyme systems, mannose phosphate isomeras
e and glutamic oxaloacetate transaminase, which appear to be diagnosti
c. Sequencing of the 18S rRNA gene in each species showed 15 nucleotid
e differences over 1815 base pairs. Digestion of the gene with the res
triction enzyme XHO I released two fragments in Placopecten and three
in Chlamys. All three techniques could be developed for management pur
poses as part of an enforcement programme to identify illegally caught
scallops.