RECOMBINATION WALKING - GENETIC SELECTION OF CLONES FROM POOLED LIBRARIES OF YEAST ARTIFICIAL CHROMOSOMES BY HOMOLOGOUS RECOMBINATION

Authors
MILLER AM SAVINELLI EA COUTURE SM HANNIGAN GM HAN ZY SELDEN RF TRECO DA
Citation
Am. Miller et al., RECOMBINATION WALKING - GENETIC SELECTION OF CLONES FROM POOLED LIBRARIES OF YEAST ARTIFICIAL CHROMOSOMES BY HOMOLOGOUS RECOMBINATION, Proceedings of the National Academy of Sciences of the United Statesof America, 90(17), 1993, pp. 8118-8122
Citations number
29
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
17
Year of publication
1993
Pages
8118 - 8122
Database
ISI
SICI code
0027-8424(1993)90:17<8118:RW-GSO>2.0.ZU;2-8
Abstract
Recombination walking is based on the genetic selection of specific hu man clones from a yeast artificial chromosome (YAC) library by homolog ous recombination. The desired clone is selected from a pooled (unorde red) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walkin g represents an efficient approach to library screening and is well su ited for chromosome-walking approaches to the isolation of genes assoc iated with common diseases.