INCORPORATION OF DINITROPHENYL DERIVATIVES OF PROTEIN-S6, PROTEIN-S13, PROTEIN-S16, AND PROTEIN-S18 INTO THE 30-S SUBUNIT OF ESCHERICHIA-COLI RIBOSOMES BY TOTAL RECONSTITUTION

This is the third paper in a series (Olah, T. V., Olson, H. M., Glitz, D. G., and Cooperman, B. S. (1988) J. Biol. Chem. 263, 4795-4800; Ols on, H. M., Olah, T., Cooperman, B. S., and Glitz, D. G. (1988) J. Biol . Chem. 263, 4801-4806) describing the use of 2,4-dinitrophenyl (DNP) derivatives of Escherichia coli 30 S ribosomal proteins to locate the positions of these proteins within the 30 S subunit by immune electron microscopy. In it we describe the derivatization of proteins S6, S13, S16, and S18 with [H-3]2,4-dinitrofluorobenzene, identify the nature of the derivatized amino acids within each protein, and demonstrate th at each DNP protein, denoted DNP-Sx, can be taken up into a reconstitu ted 30 S subunit when added to a reconstitution mixture containing 16 S rRNA and total 30 S protein depleted in Sx. We further demonstrate t hat each DNP-Sx binds within the 30 S subunit in a position identical or similar to that of the unmodified Sx protein, as judged by its meet ing one or more of the following three criteria: (i) unmodified Sx com petes with the uptake of DNP-Sx into 30 S subunits; (ii) DNP-Sx restor es functional activity to those single protein omission reconstitution particles lacking full activity; (iii) DNP-Sx induces the uptake of p roteins into 30 S subunits that depend on the presence of Sx. The four th paper in this series (Montesano-Roditis, L., McWilliams, R., Glitz, D. G., Olah, T. V., Perrault, A. R., and Cooperman, B. S. (1993) J. B iol. Chem. 268, 18701-18709), which follows this one, describes the lo calization of the DNP-Sx proteins within the 30 S subunit by immune el ectron microscopy.