HRF20 CD59 COMPLEMENT REGULATORY PROTEIN EXPRESSION IS PHENOTYPE-DEPENDENT AND INDUCIBLE BY THE HYPOMETHYLATING AGENT 5-AZACYTIDINE ON BURKITTS-LYMPHOMA CELL-LINES/

Citation
M. Kuraya et al., HRF20 CD59 COMPLEMENT REGULATORY PROTEIN EXPRESSION IS PHENOTYPE-DEPENDENT AND INDUCIBLE BY THE HYPOMETHYLATING AGENT 5-AZACYTIDINE ON BURKITTS-LYMPHOMA CELL-LINES/, Immunology letters, 37(1), 1993, pp. 35-39
Citations number
17
Categorie Soggetti
Immunology
Journal title
ISSN journal
01652478
Volume
37
Issue
1
Year of publication
1993
Pages
35 - 39
Database
ISI
SICI code
0165-2478(1993)37:1<35:HCCRPE>2.0.ZU;2-V
Abstract
We studied the expression of the 20-kDa homologous restriction factor (CD59/HRF20), a complement regulatory protein, on two subsets of blood derived B cells and on Burkitt's lymphoma lines. Both low-density (ac tivated) and high-density (resting) B cell populations expressed high levels of CD59. CD59 was detectable, however, only on a minority of ce lls or not at all on three Epstein-Barr virus (EBV)-negative BL lines (BL41, BL28 and DG75) and on clones of an EBV-positive BL line (Mutu) that phenotypically resembled resting B lymphocytes. On the other hand , CD59 was detected at high or medium levels on Mutu cells which had a lymphoblastoid cell-like phenotype. Expression of CD59 was up-regulat ed by 5-azacytidine, a drug inhibiting cytosine methylation, on CD59-n egative cell lines. Induction was accompanied by a partial hypomethyla tion in the 5' region of CD59 coding sequences.