PARTIAL-PURIFICATION AND PROPERTIES OF PECTIN LYASE FROM PENICILLIUM-EXPANSUM

Authors
SILVA DO ATTWOOD MM TEMPEST DW
Citation
Do. Silva et al., PARTIAL-PURIFICATION AND PROPERTIES OF PECTIN LYASE FROM PENICILLIUM-EXPANSUM, World journal of microbiology & biotechnology, 9(5), 1993, pp. 574-578
Citations number
NO
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
World journal of microbiology & biotechnology → ACNP
ISSN journal
09593993
Volume
9
Issue
5
Year of publication
1993
Pages
574 - 578
Database
ISI
SICI code
0959-3993(1993)9:5<574:PAPOPL>2.0.ZU;2-U
Abstract
A pectin lyase, poly(methoxygalacturonide) lyase, EC 4.2.2.10, from a culture filtrate of Penicillium expansum was partially purified 33-fol d with 7.3% yield. The enzyme was monomeric with a molecular mass of 3 6.5 kDa. The enzyme did not contain pectate lyase activity and degrade d citrus and apple pectin best at pH 7.0 and 40 to 45-degrees-C. The K (m) for citrus pectin was 9 ml-1.