CLONING AND SEQUENCING OF A NEW 15-HYDROXYPROSTAGLANDIN DEHYDROGENASERELATED MESSENGER-RNA

Authors
DELAGEMOURROUX R PICHAUD F FRENDO JL PIDOUX E GULIANA JM MOUKHTAR MS JULLIENNE A
Citation
R. Delagemourroux et al., CLONING AND SEQUENCING OF A NEW 15-HYDROXYPROSTAGLANDIN DEHYDROGENASERELATED MESSENGER-RNA, Gene, 188(1), 1997, pp. 143-148
Citations number
11
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
188
Issue
1
Year of publication
1997
Pages
143 - 148
Database
ISI
SICI code
0378-1119(1997)188:1<143:CASOAN>2.0.ZU;2-J
Abstract
NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase (type-I 15-PGDH ) inactivates prostaglandins. We recently reported an mRNA sequence co ding for a predicted isomer (PGDH(rI)) of this enzyme. The TT cell lin e, derived from medullary thyroid carcinoma (MTC), expresses mRNAs for both isomers. We report here the expression by TT cells and MTC of a third 15-PGDH related mRNA (PGDH(rII)), 241 nt shorter than type-I 15- PGDH. RNase protection assays confirmed that TT cells expressed this m RNA (PGDH(rII)). Thus different splicing patterns could be involved in the post-transcriptional regulation of type-I 15-PGDH gene in MTC.