DEVELOPMENT OF CATENARIA-ANGUILLULAE IN HETERODERA-CAJANI

The development of Catenaria anguillulae on the immobilized juveniles of Heterodera cajani was studied. Within 2 h of inoculation, the zoosp ores of C. anguillulae congregated at the oral openings of juveniles a nd in 13-15 h the germ-tube passed from the side of the stylet, growin g up to the tail region. After 1 h the mycelium became septate at regu lar intervals and swelling of the sporangia commenced, attaining full size in 40-50 min as it touched the nematode body. At this stage, seco nd septation occurred with rhizoids developing along with growing myce lium. After nearly 5 h of the second stage of septa formation, groupin g of fat globules into groups of 4-6 occurred, along with the initiati on of discharge-tubes. Precisely within 30 min, 8-12 smaller globules were rearranged in circular forms, followed by half-moon shape 17 min later. Zoospore differentiation, vesicle formation and release of zoos pores occurred within 50 min of the half-moon stage.